Abstract
Pressure effect on the survival rate of astrocytes was investigated at room temperature and 4℃. Although tissue and cells are frozen for preservation by adding cryoprotective compound, it is clarified that the survival rate of astrocytes is increased under a pressurized state at room temperature and 4℃. The optimum pressure was 0.3-1.0MPa at both room temperature and 4℃. The prominent effect was observed at 4℃ low temperature. The astrocytes died completely under normal pressure in four days at 4℃. However, under 0.3-1.0MPa, it is clarified that the survival of astrocytes increases dramatically.
