Abstract
Morphological behavior of ice crystals and cells in the tissues during the constant-temperature storage after rapid-freezing was visualized in time-series using a confocal laser scanning microscope (CLSM) with a fluorescent dye. Recrystallization of intracellular ice crystals, selectively-coarsening of fine ice crystals, was investigated quantitatively. Size and number of ice crystals were measured from the CLSM images and analyzed statistically to find frequency, average, and standard deviation of the size of ice crystals, total amount and number density of ice crystals, etc. in time-series during the storage at three different constant-temperatures. Influence of the storage-temperature of frozen tissues on these characteristics was made clear.
