Abstract
A DSC study was conducted to analyze the exotherm due to freezing of the suspension of Caenorhabditis elegans for the establishment of its cryopreservation. In the presence of Pseudomonas syringae used as an ice nucleator, temperature of freezing initiation of the nematode suspension was increased to ca. -4℃ from that obtained in the absence of the bacteria, ca. -23℃. Then, the freezing exotherm was deconvoluted into two parts, i.e., a peak part due to freezing of outside nematodes accompanied by that of inside nematodes inoculated from outside ice, and the following part due to freezing of water dehydrated from nematodes overlapped with spontaneous freezing of water inside nematodes. Results of the analysis well corresponded with those obtained with the cryomicroscopic observation.
