Cryobiology and Cryotechnology
Online ISSN : 2424-1555
Print ISSN : 1340-7902
Preservation of Desiccated Suspension Cultured Plant Cells at Various Temperatures
Masaki YAMAGUCHIHideyuki YAMAZAKIAkira KURIYAMANorio MURASE
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JOURNAL FREE ACCESS

2011 Volume 57 Issue 2 Pages 153-156

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Abstract
Suspension cultured Pogonatum inflexum and Oryza sativa cells were desiccated and preserved at various temperatures. Pogonatum cells were desiccated rapidly and Oryza cells were desiccated slowly, then preserved at various temperatures. Desiccated Pogonatum cells were successfully preserved at 26℃ for 8 weeks. Oryza cells did not survived preservation for even one day at 26℃, although the cells could be preserved for 1 week at 4℃ and for 8 weeks at -15℃. These results suggest that Oryza cells were alive just after the desiccation but produced some deleterious alternation during the preservation at higher temperature.
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© 2011 Japanese Society of Cryobiology and Cryotechnology
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