Abstract
Gametophytes of Equisetum arvense show interesting characteristics as experimental materials, for example, light dependent growth and apogamous production of sporophytic structure by exogenously applied cytokinin. Several attempts have been made for cryopreservation of the gametophytes, but successful results have not been obtained. In this study, we focused on the possibility of low temperature storage of Equisetum arvense gametophytes in unfrozen state. Our results showed the possibility of low temperature storage of this material as regrowth rate was retained after the gametophytes were exposed to low temperature (0℃) for 30 days. On the other hand, rice cells that were successfully cryopreserved in liquid nitrogen using prefreezing method did not show the positive results for the low temperature storage as regrowth ability was not recognized after exposure to 0℃ for 20 days.
