Abstract
The cryopreservation of nerve cells benefits the study with cells for medicine and poison screening. Recently, a carboxylated ε-poly-L-lysine (COOH-PLL) was found to have a remarkable effect as a cryoprotectant without any additional agents. This study investigated the effect of COOH-PLL on the post-thaw morphological changes of differentiated PC 12 cells adherent to substrate in physiological saline (PS) with different concentration of COOH-PLL. The results were compared with those of the authors' previous study for PS alone and PS with dimethyl sulfoxide, focusing on the post-thaw morphological changes of cells. The results showed that COOH-PLL is considerably effective to reduce the post-thaw morphological changes of neurites, depending on the concentration of COOH-PLL.
