Abstract
Callus was induced from an axillary bud of Ceratopteris thalictroides cultured on Murashige-Skoog medium
containing 3% sucrose and 10 μM benzyl adenine (BA). Sporophytic plants were constantly produced from
subcultured callus when transferred to BA-free medium. Callus pieces were encapsulated in arginate gel
beads and stored at -23, -5, 3, 10, 20 and 30℃. Callus tissues in gel beads stored at 20 or 30℃ for 32 days
successfully germinated and regenerated to sporophytic plants but callus tissues stored at -23 or -5℃ were
totally dead within 4 days. Gametophytes were sometimes regenerated from callus stored for rather longer
days.
