Abstract
We have studied the recovery of activity and secondary structure of bovine pancreatic ribonuclease A (RNase
A) in aqueous ionic liquid (IL) solutions including methylammonium nitrate (MAN), ethylammonium nitrate
(EAN), and propylammonium nitrate (PAN) after cryopreservation (77 K). We found that activity and
secondary structure of RNase A in aqueous IL solutions were recovered. Remarkably, concentrated aqueous
EAN solutions showed the highest recovery ability for RNase A among the studied IL concentrations. The
present results indicate that concentrated aqueous EAN solutions may have potential as a novel
cryopreservation/recovery solvent for wide variety of proteins.
(Received
