Abstract
Celosia cristata callus was induced from segments of a young seedling and two types of culture lines, one is
red pigment producing callus (red callus), the other is non-pigment producing callus (white callus), were
established by repeated selection procedure. The two types callus cells were cryopreserved by pre-freezing
method. Although white callus could be successfully cryopreserved using DMSO and glucose as
cryoprotectants, red callus was difficult for cryopreservation. By using ethylene glycol instead of DMSO red
callus could be cryopreserved although its survival rate was low. Pre-culture with high concentrations of
sucrose was effective for white callus preservation.
