Effect of the Choline-Based Ionic Liquids on Cryopreservation of Hydrated Cysts of Artemia Salina Embryos

Abstract

We investigated the effect of seven ionic liquids (ILs) on the hatching of Artemia salina hydrated cysts upon rapid cooling to －196℃. We found that the effect of an IL on the hatching of Artemia salina strongly depends on the specific combination of cation and anion. Of the ILs tested, choline acetate and choline bromide had an effect of significantly lowering the freezing damage, which may be a new promising way for the protection of freezing damage using choline-based ILs.