Abstract
Suspension-cultured cells of Oryza sativa, were cryopreserved by the pre-freezing method. Dimethyl sulfoxide (DMSO), which is often used as a cryoprotectant, is cytotoxic. In this study, we examined the effect of carboxylate polylysine (PLL) as a cryoprotectant in replacement of DMSO. It was found that cryopreservation with 5% ethylene glycol and 3.5% PLL resulted in significantly higher survival rates than that with 5% DMSO and 10% glucose. Our results indicate that PLL is effective as a cryoprotectant for cryopreservation of cultured plant cells.
