Abstract
Three pure trehalose fatty acid monoesters (TMs) with different fatty acid substitutions and their mixtures
were assessed for their ability to preserve a lactate dehydrogenase (LDH) activity during freeze/thaw
processes. 6-O-dodecanoyl trehalose (TM12) exhibited a prominent effect for preserving LDH activity.
6-O-hexadecanoyl trehalose (TM16) and 6-O-oleoyl trehalose (TM18:1), were less effective, possibly
because of the tendency for TM16 to precipitation during freeze/thaw cycles and a lower surface tension
reduction ability for TM18:1. TM18:1 was about as effective as TM16. Notably, the gradual replacement of
TM12 with TM18:1 dose-dependently reduced the ability of TMs to preserve LDH activity, and no
significant mixing effect was observed, whereas replacement with TM16 afforded the positive deviation
from the expected curve. Inhibition of TM16 precipitation during freeze/thaw processes by TM12 might
facilitate TM16 performance. The effectiveness of a mixing strategy of the stabilizer was confirmed.
