CYTOLOGIA
Online ISSN : 1348-7019
Print ISSN : 0011-4545
Cytogenetic Studies in Psilanthus ebracteolatus Hiern., a Wild Diploid Coffee Species
Ricardo A. LombelloCecília A. F. Pinto-Maglio
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Keywords: FISH, NOR, Psilanthus, rDNA site
JOURNAL OPEN ACCESS

2003 Volume 68 Issue 4 Pages 425-429

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Abstract
The genus Psilanthus is taxonomically close related to Coffea and its cytogenetic presents few studies due to the symmetry of its small chromosomes. In this work we analyzed cytogenetic features of Psilanthus ebracteolatus, a wild diploid coffee native from Ivory Coast included in the Coffee Germplasm Bank of the Instituto Agronômico de Campinas, Brazil. For heterochromatic banding assays the fluorochromes CMA3 and DAPI were used. For FISH assays the rDNA probes pTa71 and pScT7 were used. This species presented no DAPI positive bands, two CMA3 positive band pairs and one pair of NOR and one of 5S rDNA sites. Although we observed cytological similarity between the studied species and the few data presented for Psilanthus in literature, the analysis based on cytogenetic markers contributes to karyological characterization of this genus.
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© (2003), The Japan Mendel Society
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