CYTOLOGIA
Online ISSN : 1348-7019
Print ISSN : 0011-4545
Mutagenic Potential Evaluation of the Water of a River That Receives Tannery Effluent Using the Allium cepa Test System
Silvia Tamie MatsumotoMaria Aparecida Marin-Morales
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JOURNAL OPEN ACCESS

2004 Volume 69 Issue 4 Pages 399-408

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Abstract

Several studies are being conducted to assess the toxicity and cytotoxicity of water bodies receiving industrial and domestic effluents, using the Allium cepa test. To assess the toxicity and mutagenicity of water possibly contaminated with chromium, derived from tannery activities, seasonal water samplings were performed in 2001 and 2002 at five different sites along the Sapucaizinho river, Municipality of Patrocínio Paulista, State of São Paulo, Brazil. A. cepa seeds were used as the test material and were submitted to germination in waters from the different collection sites, in Milli-Q water (negative control) and in aqueous solution of chromium (positive control). For the determination of cell division rates and mitotic irregularities, slides were prepared with root tip cells according to the standard Feulgen methodology. The results showed that the collection sites most heavily compromised by chromium emission presented low mitotic indices and a higher frequency of mitotic changes such as irregular anaphases (disorganized, multipolar, laggard), cells with chromosomal adherences, cells with micronuclei, and binucleate and/or multinucleate cells.

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© (2004), The Japan Mendel Society
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