First Report on Heteromorphic NORs and Chromosome Analysis of Rolland's Demoiselle, Chrysiptera rollandi (Perciformes, Pomacentrinae) by Conventional and Ag-NOR Staining Techniques

Abstract

This is the first heteromorphic nucleolar organizer regions (NORs) and chromosome analysis of Rolland's demoiselle (Chrysiptera rollandi). Kidney cell samples were taken from five male and five female fish. Mitotic chromosome preparations were prepared directly from kidney cells. Conventional and Ag-NOR staining techniques were applied to stain the chromosomes. The results showed that the diploid chromosome number of C. rollandi was 2n=46, and the fundamental number (NF) was 50 in both males and females. The types of chromosomes were 2 large submetacentric, 2 large telocentric, 2 medium acrocentric, 32 medium telocentric, and 8 small telocentric chromosomes. In addition, the short arm of acrocentric chromosome pair 2a and the long arm of telocentric chromosome pair 6a (non-homologous chromosome) showed clearly observable NORs heteromorphic (2a6a) in both sexes and chromosome size was not related to sex. The karyotype formula for C. rollandi was:
2n (diploid) 46=L₂^sm+L₂^t+M₂^a+M₃₂^t+S₈^t