FISH Is in the Limelight Again As More Than a Cytogenetical Technique for Metaphase Chromosomes

Abstract

Fluorescent in situ hybridization (FISH) has been a powerful technique to reveal chromosomal loci and distribution of DNA sequences in metaphase chromosomes. Three-dimensional FISH (3D-FISH) has also revealed the chromosome territory of interphase chromosomes in the nucleus. Recently, 3D-FISH has played a supportive or complementary role to confirm the genome organization and topologically associated domains, which are provided by chromosome conformation capture techniques.