Differences in the Patterns of a Microsatellite Marker on Chromosomes in Four Allium Species

Abstract

This study aimed to develop molecular markers for fluorescence in situ hybridization (FISH) to examine microsatellite regions of chromosomes in four Allium species: A. ampeloprasum, A. ascalonicum, A. cepa and A. sativum using (CAA)₁₀, (GAG)₁₀, (GAA)₁₀ and (CA)₁₅ probes. The probes of (GAG)₁₀, (GAA)₁₀ and (CA)₁₅ yielded no signal. The microsatellite probe of (CAA)₁₀ hybridized to chromosomes in each species. Signals of the FISH signals were distributed across the chromosomes in A. ampeloprasum, A. ascalonicum and A. sativum but were located at chromosome termini in A. cepa.