CYTOLOGIA
Online ISSN : 1348-7019
Print ISSN : 0011-4545
Regular Article
Visualization of Chromatin Loci with Transiently Expressed CRISPR/Cas9 in Plants
Satoru FujimotoSachihiro Matsunaga
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JOURNALS OPEN ACCESS

2017 Volume 82 Issue 5 Pages 559-562

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Abstract

Labeling of specific DNA sequences in living organisms is important for understanding the mechanism of gene regulation during development, differentiation, and environmental adaptation. Here we describe a CRISPR/Cas9-based chromatin visualization system in plants. We adapted the mammalian nuclease-dead Cas9-fluorescent protein (dCas9-FP) system to a plant expression vector. Transient expression of dCas9-FP and single guide RNA (sgRNA) for telomeric sequences efficiently labeled telomere repeats in tobacco.

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© 2017 The Japan Mendel Society
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