CYTOLOGIA
Online ISSN : 1348-7019
Print ISSN : 0011-4545
Cytologia Focus
Imaging with Split Fluorescent Proteins Based on the Reconstruction of Separated Asymmetric Protein Fragments
Minato WatanabeYuki SakamotoSachihiro Matsunaga
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2018 Volume 83 Issue 4 Pages 347-350

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Abstract

Imaging techniques with split fluorescent proteins are based on the reconstruction of separated asymmetric protein fragments. Although each fragment alone has no fluorescence, a reconstructed protein after the association of its constituent fragments recovers its fluorescence. In the case of the split-GFP assay, GFP is divided between the 10th and 11th β sheets to generate two asymmetric fragments, GFP1–10 and GFP11. GFP11 is a small tag that minimally disturbs the localization and dynamics of a fused protein. Thus, the split fluorescent protein assay enables analyses of protein localization patterns, protein–protein interactions and the topology of membrane proteins.

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© 2018 The Japan Mendel Society
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