2019 Volume 84 Issue 1 Pages 89-92
Cytological investigations have been carried out in one wild accession of Anemone rupicola Cambess. collected from the cold deserts of Ladakh division of Jammu & Kashmir India. The chromosome count of 2n=16 was observed and is the first chromosome record for the species from India. Meiotic analysis showed structural heterozygosity for reciprocal translocations as indicated by the presence of multiple associations of four chromosomes. Detailed meiotic analysis showed Pollen Mother Cells (PMCs) with laggards (32.87%) and chromatin bridges (21.91%) at anaphases I, II (A I, II)/telophases I, II (T I, T II), leading to abnormal microsporogenesis. Consequently, abnormal sporads such as triads (37.14%) and polyads (28.57%) were formed. Structural heterozygosis and other associated abnormal meiotic irregularities in the species showed considerable pollen sterility (32.20%) which could be attributed to the presence of ring/chain shaped multivalent in PMCs.
