Assessment of Oxaliplatin and Carboplatin on Some Attributes of Cell Division in Lathyrus sativus L.

Abstract

The effect of alkylating anti-cancerous drugs oxaliplatin and carboplatin were assessed on germinating seedlings of Lathyrus sativus L. Concentrations used for oxaliplatin were 1 µM, 10 µM, 20 µM and 30 µM respectively; and 100 µM, 200 µM, 300 µM and 400 µM for carboplatin. The radicle length and mitotic index decreased with the increasing concentration of the drugs. Polyploidy was induced on germinating roots by pre-treatment (8 h) with 0.5% colchicine. The inhibitory effect of the drugs on the induced polyploidy was evident with the increasing doses of the drugs. In vitro callus growth in different doses of the drugs in media was measured by taking the weight and area of the callus. The objective of the work was to assess the effectiveness of alkylating drugs on the plant-based model to develop a plant-based model system for the primary screening of anti-cancerous drugs.