Abstract
Podophyllotoxin in concentrations of 50 mg/L in the nutrient solution of tissue cultures produces a complete metaphase block.
At 50mg/L and 500mg/L the cell shows other toxic effects. Cells become rounded, blebbed, vacuolate, and pycnotic. The severity of response increases with increased concentration of podophyllotoxin and duration of exposure. The type of response and its severity vary with the type of tissue studied. The mouse tissues used in these experiments were: normal fetal skin, testis, mammary adenocarcinoma, teratoma, reticulum cell sarcoma and L-sarcoma.
