Abstract
Division of the generative cell which occurs in the pollen tube was studied in seven species of angiosperms. Observations were made in vivo with use of the phase-contrast microscope, Fixed and stained preparations were also used to check the results from observations in vivo.
The chromosomes are arranged in the equatorial plane in metaphase, The daughter chromosomes are regularly separated in anaphase towards the both poles. The spindle can be well observed in vivo as a transparent body, The phragmoplast and the cell plate are formed in the course of cytokinesis, the cell plate developing centrifugally. The phragmoplast and also the cell plate begin to degenerate after they have reached the maximum development.
In fixed and stained preparations the spindle and the phragmoplast mani-fest to contain pentosenucleic acid. The cell plate region does not contain pentosenucleic acid and also does not show a positive staining reaction for polysaccharides.
Division of the generative cell in the pollen tube is considered to occur essentially in the same manner as in other typical plant cells, although the cell plate is of a degenerating character.
