Abstract
1) The effect of 2 methyl amino 1.3 diaza-azulene on the mitotic cells of Tradescantia was investigated in vivo by application of the agar plate method.
2) In concentrations higher than 5×10-3M, nuclei with doubled chromosome number are induced, when the cells are treated in prophase and prometaphase, while bi-nucleate cells are induced, when treated in metaphase.
3) In a range of 1×10-3 M concentration, the atractoplast is suppressed in its function when the cells are treated in pro-metaphase but can develop into phragmoplast substance. As results of combinations of these phenomena, multi-nucleate cells with multi-septa, appear in telophase.
4) From the results of these experiments, the abnormal behaviour of the mitosis is analysed into two steps: one is the suppression of the atractoplast, and the other, the deformation of the phragmoplast. Also, the relationship between the stage of mitosis at the beginning of the treatment and the types of abnormal mitosis is discussed.
