A New Technique for the Microscopic Observation of Colored Particles with Opal Glass

Abstract

In this report, is presented a new method for the microscopic observation of colored particles in tissues or cells, in which opal glass is placed in a position directly under a slide glass to illuminate a sample with completely diffuse light. It was demonstrated that, under diffuse-light illumination, we have practically no image due to the difference of refractive index such as those of colorless boundaries, so that we can observe only the images of colored particles with no interference of the images of the boundaries. Furthermore, the images of colored particles are sharply defined with no deformation under diffuse-light illumination, whereas they are obscure and more or less deformed under normal illumination. The principle of the new technique is described together with the characteristics in the microscopic observations of colored particles.