Abstract
1. By using the dilute acridine orange solution as a fluorochrome, the developmental process of the spindle body and the chromosomal fibers are investigated in vivo in pollen mother cells of Lilium longiflorwn; through such specific treatment secondary fluorescence has been demonstrated.
2. The transition of nuclear substances into atractoplasm, the nature of the chromosomal fibers and the fibrillar structure of the spindle body have been discussed based on their fluorescent tints.
3. The applications of this technique on mitotic cells are excellent to elucidate the relationships between the morphological changes of cellular elements and the chemical ones of their constituents in a mitotic cycle.
