In Vitro Cultivation of Rabbit Ova Following In Vitro Fertilization in Tubal Fluid

Abstract

A total of 207 rabbit ova were inseminated in vitro in tubal fluid under mineral oil using capacitated spermatozoa recovered from the uterus 12 hours after mating. About 20 hours after the transfer for cultivation, 123 (59.4%) of them were at the 2- to 4-cell stages. Sixty-five of the fertilized ova were cultivated for two or three days after insemination in vitro, and 21.5% of them could be reached at over the 16-cell stage.