Cytochemical Electron Microscopic Studies on the Kinetochore of Pollen Mother Cells in Tradescantia reflexa

Abstract

Cytochemical studies were made on the kinetochore of chromosomes in the first meiotic metaphase and anaphase of pollen mother cells of Tradescantia reflexa. The kinetochore of T. reflexa is ball- or cup-shaped and about 700 nm in diameter. It seems to contain DNA, RNA and basic proteins judging from the effect of uranyle-EDTA treatment, RNase digestion and PTA-staining. The location of these chemical components were also examined.