Abstract
In the meristernatic root cells of alfalfa seedlings exposed to -1, 5°C, new spherosomes, 0, 1-0, 4μm in diameter, are observed each one within the terminal end of an endoplasmic reticulum (RE) segment. A two-unit membrane envelope surround these new organelles and the external membrane of their envelope is continuous with the RE. Those new spherosomes are filled with an osmiophilic and electron dense material. This osmiophilic material within the new sphero-somes is insoluble in petroleum ether, a solvent of neutral lipid, and soluble in ethylic ether: ethanol, 2: 1, a solvent of polar lipids.
