1995 Volume 60 Issue 1 Pages 85-91
The distribution patterns of microtubules (MTs) in the mitotic cells of Spirogyra were examined by immunofluorescence microscopy. Some of the cortical MTs and MT-fibrils in the cytoplasmic strands at interphase seemed to be organized into MT-belts at prophase. The MT-belts fused side by side, decreased in length and developed into spindle-MTs at metaphase. During polar separation at anaphase, the shape and size of the half-spindles remained almost unchanged. Considerable amounts of polar MTs were seen between the two separating half-spindles. It seems possible that these polar MTs, which could consist of pairs of antiparallel MTs, might push the poles apart by a sliding mechanism.