Physical Mapping of 18S rDNA by Fluorescence in situ Hybridization (FISH) in the Three Species of the Genus Paris L., Liliaceae

Abstract

18S ribosomal RNA gene (rDNA) loci were mapped on chromosomes of Paris tetraphylla A. Gray, P. verticillata M. v Bieberstein and P. polyphylla Smith, Liliaceae by using fluorescence in situ hybridization (FISH). The 18S rDNA probes were prepared from the interphase nuclei by the method during the polymerase chain reaction (PCR). Chromosome numbers of all the examined plants were 2n=10. Haploid genomes of P. tetraphylla and P. verticillata were consisted of three metacentric chromosomes, a submetacentric chromosomes and an acrocentric satellite chromosome. One 18S rDNA locus was detected at the second constriction of the acrocentric chromosome. On the other hand, a haploid genome of P. polyphylla was consisted of three metacentric chromosomes and two acrocentric chromosomes. Two 18S rDNA loci were detected at the second constriction of both acrocentric chromosomes.