Abstract
Morphogenetic response of triticale callus cultures was studied after supplement of the herbicide Stomp 330 into the regeneration nutrient medium. It is found, that the regenerative ability is not affected at the lowest herbicide concentration. However, the higher doses reduce regenerative ability at different rates depending on the genotype. Rhizogenesis is completely suppressed. The necrotic calli increased and the fresh, unorganized growing calli reduced. Numerous abnormalities are observed in the treated tissues. Considerable part of the cells, especially those, situated in intensively proliferating zones are enlarged and vacuolized. Abnormalities as chromatin pycnosis and micronuclei are observed. The frequency of these abnormalities is proportional to the herbicide concentration. It is established, that Stomp 330 exerts strong C-mitotic effect in the callus cells, which is demonstrated by high frequency of polyploid cells.
