Application of Flow Cytometry to Translational Study of Target-based Therapy.

Abstract

Molecular correlative studies are key to the identification and maximization of benefit of target-based therapy. Flow Cytometry is a powerful tool for examining mode of action of Target-based therapy. DNA histogram and its mathematical approach allow us to elucidate the antitumor effects of cell cycle regulators. Binding of antibody and tumor cells is directly evaluated by FCM. Intracellular signaling pathway of Target-based drugs in individual cells could be monitored by FCM analysis using phosphospecific antibodies. The correlative studies using FCM allow us to develop the individualized medicine for cancer patients.