2004 Volume 14 Issue 2 Pages 23-32
Clinical and research laboratories are getting to face an increasing number of requirements on the validation and standardization of their procedure and machinery reliance. Flowcytometric analysis is one of those.On the view of the flowcytometer, it should be managed to be stay reliable with some standardized procedure,however its method for calibration and validation is not widely accepted. We have evaluated several factors for the machinery calibration to settle reliability as follows; (1)utility of the CaliBRITETM beads with FACSCompTM software on many Becton Dickinson(BD) cytometers as general practice; (2) utility of the DNA QC Particles tovalidate the linearity for the cell cylce analysis; (3) utility of the SPHEROTM Rainbow QC Kit to be reliable of stability of the each detector on the light intensity and signal resolution. The result shows that ; (1)the CaliB-RITETM beads provide information on signal separation, compensation, the sensitibity of each detector and the flow stabilizastaion, and it settels appropriate instrument setting for peripheral blood specimens. And those information can be monitored by the Levy-Jennings charts as a time-being data. (2) The DNA QC Particles can be validate the linearlity of the FL2 channel detector and its parameters. It also can be separate the single cell from the doublets and others. Actual proof of the CV is possible as a result. (3) The QuantiBRITETM beads can be used for the quantification of the antigen-antibody reaction, and the SPHEROTM Rainbow QC Kit is useful for the evaluation of the signal intensity and the lineality for the quantification.
