Cell Cycle Analysis using Flow Cytometry Ⅲ

― Stathomokinetic Analysis ―

Abstract

The stathomokineses method was presented as the cell cycle analysis to determine cell cycle parameters. In this method, all of the relationships are derived from an equation that n(t, τ) =αN₀exp(αt)exp(ατ), where n(t, τ) is cell density of a phase τ at time t, N₀ is the initial cell number at time t = 0, T_c is cell-cycle time and α = ln2 / T_c. The stathomokinetic agents, colchicines or demecolcine (Colcemid), are added in a cell population to block cell cycle progression at the M phase. The plotting of G₁ or G₂/M cell fractions against time reveals the cell cycle parameters. A computer program of cell cycle analysis of unconstrained-type is presented to help the stathomokinetic analysis.