The Rapid immunostaining Technique using a slide seal and microwave.

Abstract

There have been many reports of Rapid immunostaining Technique with Microwave（MW）treatment, which promote antigen-antibody reaction. There remains a problem of temperature rise and evaporation of antibody solution during MW irradiation. Here we propose a method of MW treatment using a slide seal, with which reaction solution is enclosed on a glass slide. The sealed glass slide was irradiated with MW in a chamber that is filled with water. This method was applied to immunoperoxidase staining of cytokeratin in colon cancers. A satisfactory result was obtained after MW treatment for 3 minutes with anti-cytokeratin antidody and subseguent MW treatment with Simplestain solution for 3 minutes. This method is applicable to intraoperative diagnosis.