2010 Volume 20 Issue 2 Pages 1-4
The different methods for sperm preparation are used routinely to obtain a population of highly motile sperm for assisted reproductive technology. But, the study of chromosomal abnormalities in highly motile sperm obtained by sperm preparation is under debate. Using fluorescence in situ hybridization, we evaluated the fre quency of numerical sex chromosomal abnormalities in highly motile sperm obtained by swim-up method in the current study. Semen samples of 27 individuals, diagnosed as normal according to criterion of World Health Organization, were analyzed. These samples are classified as follows : swim-up group ; motile sperm obtained by swim-up method, pellet group ; sperm obtained by pellet, no treatment group ; no treatment sperm. At least 15000 sperm were analyzed for each sample. In swim-up group, the ratio of X-, Y-bearing, and sex chromoso mal numerical abnormality sperm were 47.6%, 52.2%, and 0.17%, respectively. In pellet group, the ratio of X-, Y bearing, and sex chromosomal numerical abnormality sperm were 52.2%, 47.3%, and 0.50%, respectively. In no treatment group, the proportion of X-, Y-bearing, and sex chromosomal numerical abnormality sperm were 50.0%, 49.7%, and 0.26%, respectively. There were not significant differences in the proportion of X- and Y bearing sperm among each group. The proportion of sex chromosomal numerical abnormality sperm in swim up group was significantly lower than that of pellet group and no treatment group. These results suggest that swim-up method can reduce the proportion of sperm with sex chromosomal numerical abnormality, but not absolutely. Therefore, it is necessary to study sperm selection more.
