2012 Volume 22 Issue 1 Pages 7-11
At our hospital, we started collection of peripheral blood stem cells (PBSC) in 1999, and began to examine the CD34 phenotype of the PBSC using the Stem-Kit (Beckman-Coulter) in 2002. We compared the CD34-positive rate between our data on the day of collection or on the following day and those of the inspecting company on the following day. The CD34-positive rate differed slightly depending on the type and clone of the anti-CD34 antibody used. When a class Ⅲ antibody of anti-CD34 was used, such as clone 581 or 8G12 as in this study, the correlation coefficient of the CD34- positive rate between our hospital and the inspecting company was r=0.996, posing no problems. Furthermore, there was also no influence of either the flow cytometric equipment used or of examination on the day after the harvest.
