The possibility of cell cycle analysis with image cytometry

Abstract

Two-color fluorescence in situ hybridization (FISH) with chromosome enumeration DNA probe (CEP) specific to chromosomes 7 and 11, respectively was applied to breast cancer cell and breast cancer cell line CAL-51 to examin whether the fluorescence intensity of FISH spots is associated with the cell cycle progression. The fluorescence counts and intensity of FISH spots was quantitatively measured with a technique of image cytometry for individual cell in relation to the cell cycle. The fluorescence count of FISH spots was able to classified diploidy or anueploidy cell cycle. And the fluorescence intensity of FISH spots was two-times greater in G2 cells than in G1 cells. This image cytometry for FISH spots in the relation to the cell cycle adds a new dimension to genetics.