成人T 細胞白血病（ATL）におけるフローサイトメトリー解析法の確立とその臨床応用 〜12カラーの病態解析から4カラーの臨床検査まで〜

石垣 知寛; 小林 誠一郎; 在家 裕司; 渡辺 恵理; 佐藤 奈津子; 大野 伸広; 渡辺 信和; 内丸 薫; 東條 有伸; 中内 啓光

doi:10.18947/cytometryresearch.24.2_33

review

Establishment of flow cytometric methods for evaluation of adult T-cell leukemia and their clinical application

Tomohiro Ishigaki, Seiichiro Kobayashi, Yuji Zaike, Eri Watanabe, Natsuko Sato, Nobuhiro Ohno, Nobukazu Watanabe, Kaoru Uchimaru, Arinobu Tojo, Hiromitsu Nakauchi

Author information

Tomohiro Ishigaki
Division of Stem Cell Therapy, The Institute of Medical Science, The University of Tokyo (IMSUT)
Clinical FACS core laboratory, The Institute of Medical Science, The University of Tokyo
Seiichiro Kobayashi
Division of Molecular therapy, The Institute of Medical Science, The University of Tokyo
Yuji Zaike
Department of Laboratory Medicine, Research Hospital, IMSUT.
Eri Watanabe
Clinical FACS core laboratory, The Institute of Medical Science, The University of Tokyo
Natsuko Sato
Clinical FACS core laboratory, The Institute of Medical Science, The University of Tokyo
Nobuhiro Ohno
Department of Hematology and Oncology, Research Hospital, IMSUT.
Nobukazu Watanabe
Clinical FACS core laboratory, The Institute of Medical Science, The University of Tokyo
Kaoru Uchimaru
Department of Hematology and Oncology, Research Hospital, IMSUT.
Arinobu Tojo
Division of Molecular therapy, The Institute of Medical Science, The University of Tokyo
Department of Hematology and Oncology, Research Hospital, IMSUT.
Hiromitsu Nakauchi
Division of Stem Cell Therapy, The Institute of Medical Science, The University of Tokyo (IMSUT)

Keywords: Adult T-cell leukemia/lymphoma, HTLV-1, Flow cytometry, HAS-Flow

JOURNAL FREE ACCESS

2014 Volume 24 Issue 2 Pages 33-39

DOI https://doi.org/10.18947/cytometryresearch.24.2_33

Details

Abstract

We review here flow cytometric gating methods, which were newly established and named “HAS-Flow,” to analyze ATL (adult T-cell leukemia/lymphoma) cells. Using CD7 vs. CD3 or CADM1 plots of CD4-positive lymphocytes, HTLV1- infected ATL cells can be specifically separated from other normal T-cells. These plots are different among subtypes of ATL, and reflect the disease progression from HTLV-1 infection through indolent ATL to aggressive ATL. Moreover, the change of the plots after chemotherapy could distinguish chemo-sensitive cases and predict prognosis. We are now advancing their clinical applications. For example, recurrence of ATL and engraftment after HSCT (hematopoietic stem cell transplantation) can be monitored simultaneously by combining HAS-Flow with HLA-Flow, which can analyze mixed chimerism among various cell populations after HSCT.

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