Mouse model of mature B-cell lymphoma using in vitro retroviral gene-transduction systems

Abstract

Accumulation of genetic aberrations causes mature B-cell lymphoma. Recent advances in high-throughput technology, including next generation sequencing, have made it possible for us to understand the landscape of genetic abnormalities in lymphoma; however, function-based synergistic analyses of candidate genes for lymphoma development have not been available, except for the transgenic mouse model. This type of functional analyses is essential to understand collaborative pathways leading to malignant transformation and is also important to provide a corroborative evidence for a rationale combination therapy. Therefore, we established an in vitro culture system that allowed multiple gene transduction into normal murine mature B-cells and in vivo transplantation model. In this review, we demonstrate how we explored the molecular mechanisms of B-cell lymphoma development and summarize the recent progress in molecular understandings of the B-cell lymphoma, along with future directions for lymphoma research.