2020 Volume 30 Issue 1 Pages 1-
The clinical effectiveness of intraoperative flow cytometry (iFC) for glioma has been studied by several researchers. However, the studies of its analytic validity are yet few. Particularly, the repeatability, which is a very basic characteristic of analytic validity, cannot be evaluated in principle because the specimen becomes a cell suspension by cell dispersion. Therefore, in this study, a stochastic measurement model was developed and a surrogate index to indirectly evaluate the repeatability was defined. In addition, the cell dispersion function of Celltac PEAK, a clinical flow cytometer dedicated to iFC, was evaluated by this method. As a result, the repeatability of the cell dispersion function was interval-estimated as [0.2%, 1.7%] with standard deviation.
