Monoclonal antibody production technology optimised for cytometry: from theory to practice.

Abstract

Monoclonal antibodies are an essential biological material for the development of test drugs in medical biology and pharmaceuticals, but their production is fraught with numerous difficulties. In particular, the limited availability of commercial monoclonal antibodies suitable for flow cytometry analysis has slowed the progress of research.

The MIHS method developed by our group is a state-of-the-art monoclonal antibody production technique that combines hybridoma technology with flow cytometry. This method is an innovative technique for targeting antigens with physiological structures and is particularly useful for fl ow cytometric analysis. In this review, the MIHS method is presented, which can be easily implemented by laboratories skilled in the operation of fl ow cytometers.