Journal of Environment and Safety
Online ISSN : 2186-3725
Print ISSN : 1884-4375
ISSN-L : 1884-4375
Original article
Gene expression analysis of Mysid Crustacea (Americamysis bahia) exposed to Triclosan and Triclocarban using DNA microarray
Masaya UchidaSonoko MiuraMasashi HiranoAyako InokuchiRyoko YamauchiRemi YoshiduHiroshi NakamuraYoshihiro KagamiTeruhiko KusanoMinoru KogaKoji Arizono
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2013 Volume 4 Issue 3 Pages 177-183

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Abstract

 Triclosan (TCS) and Trichlorocarban (TCC) are widely used as antibacterial agent in various industrial products,such as textile goods, soap, shampoo, liquid toothpaste and cosmetics, and often detected in wastewater effluent. The aim of this study was to investigate toxicologically significant effects of TCS and TCC exposure on gene expression in mysid (Americamysis bahia) using DNA microarray. Juvenile mysid were exposed to 0.5 µg/L of TCS and 0.05 µg/L of TCC for 24 hours, and mRNA expression profiles in their whole bodies were analyzed. We identified statistically significant (p<0.05) regulation (expression ratio of exposed to control > 3) in 312 genes responding to 0.5 µg/L of TCS and 264 genes responding to 0.05 µg/L of TCC. There were merely 231 genes found responding to both exposures of TCS and TCC. The up-regulation in response to TCS and TCC was observed in vitellogenin and clottable protein. These genes are expressed more than 30 times in both experimental plots, which indicates the applicability of them in serving as biomarkers of antibacterial agents in mysid. These results suggested that endocrine disrupting effect of TCS and TCC would be potent for marine crustacean.

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© 2013 Academic Consociation of Environmental Safety and Waste Management, Japan
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