Development of IVV method into drug discovery

Abstract

We have originally developed the so-called in vitro virus (IVV) method as a more stable and efficient tool for analyzing protein functions. The IVV method is applicable for analyzing and screening protein-protein interactions, transcription factors, RNA binding proteins, bioactive peptides, drug-target proteins, and antibodies using protein, DNA, RNA, drug and antigen as baits, and a randomly primed cDNA library. We further developed a large-scale and high-throughput IVV screening system utilizing a biorobot and microfludic chip.