Abstract
The efficient development of safe, orally administered drugs requires accurate prediction of pharmacokinetics in the patient’s intestine at the preclinical stage. However, the current in vitro intestinal pharmacokinetic assay systems have several drawbacks, including species differences and low（or no） expression of drug-metabolizing enzymes and transporters. Therefore, it is expected that intestinal epithelial cells generated from human iPS cells（human iPS cell-derived intestinal epithelial cells） will be used to evaluate the pharmacokinetics of drugs in the human intestinal tract. There are two main methods for generating human iPS cell-derived intestinal epithelial cells: those involving three-dimensional culture（intestinal organoids） and those involving consistent two-dimensional culture. In this review, we discuss the pharmacokinetic applications of human iPS cell-derived intestinal epithelial cells generated by each method.
