Abstract
Cancer cell lines are essential research tools in drug discovery. They are widely used and contribute to the development of many drugs, especially in preclinical anticancer pharmacology research. Cancer cells are very easy to handle because they have acquired the ability to proliferate infinitely and grow logarithmically in culture dishes. In the early days of the use of cancer cell lines, many cancer cell lines based on different tissues and organs of origin were established and registered in cell banks and made available. On the other hand, cancer cell lines, which are mainly used in two-dimensional culture, have problems such as the fact that they cannot be distinguished from cancer cells in living organisms because only cells that proliferate rapidly in culture dishes are selected. However, the technology is expected to be further utilized since it is very useful for detailed analysis of cancer cells and for genetic modification such as genome editing.
