Comprehensive protein-protein interaction analysis (proteorne analysis) using “in vitro virus” and its application for drug target exploration

Abstract

In vitro virus(IVV) is an assignment molecule in which the genotype molecule (mRNA) binds to the phenotype molecule (protein). We have demonstrated that an IVV selection system is useful for the comprehensive analysis of protein-protein interactions (proteome analysis). We are studying to apply the IVV selection system to the analysis of drug-protein interactions to explore the drug target proteins or to understand the mode of action of drugs. In this review, we describe about the structure and the property of IVV. The application of the IVV selection system for proteome analysis and the analysis of drug-protein interactions is also explained.