Abstract
A new dosage format(ETOP-CH)which comprised activated carbon particles adsorbing etoposide was examined for anticancerous effects on the metastases in paraaortic lymph nodes in mice. Eight days after inoculation of P388 leukemia cells into the left hind foot-pad of mice (donors), when cancer metastases had been established in the paraaortic nodes, etoposide at 5 mg/kg was administered intraperitoneally in the form of ETOP-CH or etoposide aqueous solution. Ten days after inoculation, a tissue suspension of paraaortic nodes and left iliac nodes taken from each donor mouse was intraperitoneally transferred individually to a new mouse (recipient). Because it is known that the recipients' survival time correlates inversely to the implanted P388 leukemia cell number, the therapeutic effects of treatment on the metastases in the donors' paraaortic nodes was compared through the comparison of survival time of the recipients. The mean survival time of the recipients was statistically significantly (by Student's τ test, P<0.05) long in the group given ETOP-CH than in the groups given etoposide aqueous solution and given activated carbon without etoposide, as well as than in the non treatment group.
