The vaccine effect of anti-idiotypic monoclonal antibody in the melanoma antigenic system

Abstract

The usefulness of anti-idiotypic(Id) antibodies was investigated in the malignant melanoma antigenic system. Anti-Id monoclonal antibodies (MoAbs) were established to MoAb M2590 (1gM) which recognized the glycolipid GM3 on the syngeneic Melanoma B16 cells. Anti-Id MoAb D704 had a specificity only to MoAb M2590, recognized the private idiotype, and furthermore was serologically supposed to have an internal image of Ag. In vivo experiments were performed to see the anti-Id MoAb effect to melanoma cells transplanted black mouse. Internal image-bearing anti-Id MoAb D704 showed the vaccine effect against transplanted syngeneic melanoma cells. Therefore, internal image bearing anti-Id antibodies may be useful for induction of a host's immune response against tumors.